Recombinant:Article Title: Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-p63 Santa Cruz Biotechnology Cat# sc-8431; RRID: AB_628091; clone 4A4 Rabbit polyclonal anti-PAX6 Covance Research Products Inc Cat# PRB-278P; RRID: AB_291612 Rabbit polyclonal anti-E-cadherin Santa Cruz Biotechnology Cat# sc-7870; RRID: AB_2076666 Goat polyclonal anti-K12 Santa Cruz Biotechnology Cat# sc-17098; RRID: AB_639266 Mouse monoclonal anti-MUC5AC Santa Cruz Biotechnology Cat# sc-33667; RRID: AB_627973; clone CLH2 Mouse monoclonal anti-K13 Abcam Cat# ab16112; RRID: AB_302267; clone AE8 Mouse monoclonal anti-MUC4 Abcam Cat# ab52263; RRID: AB_881163; clone 8G-7 Mouse monoclonal anti-K4 Abcam Cat# ab9004; RRID: AB_306932; clone 6B10 Mouse monoclonal anti-K7 Abcam Cat# ab9021; RRID: AB_306947; clone RCK105 Rabbit polyclonal anti-K19 Abcam Cat# ab15463; RRID: AB_2281021 Mouse monoclonal anti-K10 Dako Cat# M7002; clone DE-K10 Rabbit monoclonal anti-phospho-EGF receptor (Tyr1068) Cell Signaling Technology Cat# 3777; RRID: AB_2096270; clone D7A5 Rabbit monoclonal anti-EGF receptor Cell Signaling Technology Cat# 4267; RRID: AB_2246311; clone D38B1 Mouse monoclonal anti-b-actin Sigma-Aldrich Cat# 5441; RRID: AB_476744; clone AC-15 Mouse monoclonal anti-SSEA-4 PE conjugate BioLegend Cat# 330406; RRID: AB_1089206; clone MC-813-70 Mouse monoclonal anti-CD104 Alexa Fluor 647 conjugate BD Biosciences Cat# 624024; clone 450-9D Mouse monoclonal anti-CD200 PE-Cy7 conjugate BD Biosciences Cat# 624052; clone MRC OX-104 Donkey anti-mouse IgG Alexa Fluor 488 conjugate Life Technologies Cat# A-21202; RRID: AB_141607 Donkey anti-rabbit IgG Alexa Fluor 594 conjugate Life Technologies Cat# A-21207; RRID: AB_141637 Donkey anti-goat IgG Alexa Fluor 568 conjugate Life Technologies Cat# A-11057; RRID: AB_142581 Donkey anti-rabbit IgG horseradish peroxidase conjugate GE Healthcare Cat# NA934-100Ul; RRID: AB_772206 Sheep anti-mouse IgG horseradish peroxidase conjugate GE Healthcare Cat# NA931-100Ul; RRID: AB_772210 Biological samples Research-grade human corneoscleral tissue SightLife https://sightlife.org/ Chemicals, peptides, and recombinant proteins iMatrix-511 (LN511E8) Nippi Cat# 892012 FBS Life Technologies Cat# 12483-020 Mitomycin C Kyowa Hakko Kirin N/A Dispase II, powder Life Technologies Cat# 17105-041 (Continued on next page) e1 Cell Reports 34, 108715, February 2, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER TrypLETM Express Enzyme (1X), phenol red Thermo Fisher Scientific Cat# 12605010 KnockOutTM Serum Replacement Life Technologies Cat# 10828-028 Sodium pyruvate Life Technologies Cat# 11360-070 Non-essential amino acids Life Technologies Cat# 11140-050 L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin-streptomycin solution Life Technologies Cat# 15140-122 2-Mercaptoethanol Life Technologies Cat# 21985-023 Monothioglycerol Wako Cat# 195-15791 Y-27632 Wako Cat# 034-24024 Recombinant human EGF R&D Systems Cat# 236-EG Recombinant human KGF Wako Cat# 112-00813 Recombinant human TGFa R&D Systems Cat# 239_A Recombinant human Amphiregulin R&D Systems Cat# 262-AR B27TM supplement Life Technologies Cat# 17504-044 QIAzol Lysis Reagent QIAGEN Cat# 79306 4% paraformaldehyde phosphate buffer solution Wako Cat# 163-20145 Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 TritonTM-X 100 Sigma-Aldrich Cat# T8787 Hoechst 33342 Wako Cat# 346-07951 RIPA buffer (10x) Nacalai Tesque Cat# 08714-04 PhosSTOPTM (phosphatase inhibitor) Sigma-Aldrich Cat# 4906845001 StemProTM AccutaseTM Cell Dissociation Reagent Life Technologies Cat# A11105-01 Methanol Wako Cat# 131-01826 10% formaldehyde neutral buffer solution Wako Cat# 062-01661 Rhodamine B Wako Cat# 180-00132 Critical commercial assays SuperScriptTM III First-Strand Synthesis System for qRT-PCR Life Technologies Cat# 18080051 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 ECLTM Prime Western Blotting Detection Reagent GE Healthcare Cat# RPN2236 BD Cytofix/CytopermTM Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 PAS staining kit MERCK KGaA Cat#101646 ELISA Kit for Mucin 5 Subtype AC (MUC5AC) USCN Cat# SEA756Hu Experimental models: cell lines Human: iPS cell line 1383D2 Center for iPS Cell Research and Application N/A Human: iPS cell line 201B7 RIKEN Bio Resource Center Cat# HPS0063; RRID: CVCL_A324 Human: iPS cell line YZWJs524 Center for iPS Cell Research and Application N/A Mouse: NIH 3T3 cell line N/A N/A Oligonucleotides TaqMan probes were listed in Table S1 Life Technologies Cat# 4331182 Software and algorithms AxioVs40 version 4.8.2.0 Carl Zeiss N/A (Continued on next page) Cell Reports 34, 108715, February 2, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER 7500 Software version 2.0.6 Thermo Fisher Scientific RRID: SCR_014596; https://www. thermofisher.com/us/en/home/technicalresources/software-downloads/appliedbiosystems-7500-real-time-pcrsystem.html WorkOut 2.5 version 1.0.0.0 Dazdaq Solutions N/A Image Lab Software version 4.1 Bio-Rad RRID: SCR_014210; http://www.bio-rad. com/en-us/sku/ 1709690-image-lab-software?ID=1709690 Cell Sorter Software version 2.1.5 SONY N/A JMP pro version 14.1.0 SAS RRID: SCR_014242; https://www.jmp.com/ en_us/software/ predictive-analytics-software.html
Lysis:Article Title: Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-p63 Santa Cruz Biotechnology Cat# sc-8431; RRID: AB_628091; clone 4A4 Rabbit polyclonal anti-PAX6 Covance Research Products Inc Cat# PRB-278P; RRID: AB_291612 Rabbit polyclonal anti-E-cadherin Santa Cruz Biotechnology Cat# sc-7870; RRID: AB_2076666 Goat polyclonal anti-K12 Santa Cruz Biotechnology Cat# sc-17098; RRID: AB_639266 Mouse monoclonal anti-MUC5AC Santa Cruz Biotechnology Cat# sc-33667; RRID: AB_627973; clone CLH2 Mouse monoclonal anti-K13 Abcam Cat# ab16112; RRID: AB_302267; clone AE8 Mouse monoclonal anti-MUC4 Abcam Cat# ab52263; RRID: AB_881163; clone 8G-7 Mouse monoclonal anti-K4 Abcam Cat# ab9004; RRID: AB_306932; clone 6B10 Mouse monoclonal anti-K7 Abcam Cat# ab9021; RRID: AB_306947; clone RCK105 Rabbit polyclonal anti-K19 Abcam Cat# ab15463; RRID: AB_2281021 Mouse monoclonal anti-K10 Dako Cat# M7002; clone DE-K10 Rabbit monoclonal anti-phospho-EGF receptor (Tyr1068) Cell Signaling Technology Cat# 3777; RRID: AB_2096270; clone D7A5 Rabbit monoclonal anti-EGF receptor Cell Signaling Technology Cat# 4267; RRID: AB_2246311; clone D38B1 Mouse monoclonal anti-b-actin Sigma-Aldrich Cat# 5441; RRID: AB_476744; clone AC-15 Mouse monoclonal anti-SSEA-4 PE conjugate BioLegend Cat# 330406; RRID: AB_1089206; clone MC-813-70 Mouse monoclonal anti-CD104 Alexa Fluor 647 conjugate BD Biosciences Cat# 624024; clone 450-9D Mouse monoclonal anti-CD200 PE-Cy7 conjugate BD Biosciences Cat# 624052; clone MRC OX-104 Donkey anti-mouse IgG Alexa Fluor 488 conjugate Life Technologies Cat# A-21202; RRID: AB_141607 Donkey anti-rabbit IgG Alexa Fluor 594 conjugate Life Technologies Cat# A-21207; RRID: AB_141637 Donkey anti-goat IgG Alexa Fluor 568 conjugate Life Technologies Cat# A-11057; RRID: AB_142581 Donkey anti-rabbit IgG horseradish peroxidase conjugate GE Healthcare Cat# NA934-100Ul; RRID: AB_772206 Sheep anti-mouse IgG horseradish peroxidase conjugate GE Healthcare Cat# NA931-100Ul; RRID: AB_772210 Biological samples Research-grade human corneoscleral tissue SightLife https://sightlife.org/ Chemicals, peptides, and recombinant proteins iMatrix-511 (LN511E8) Nippi Cat# 892012 FBS Life Technologies Cat# 12483-020 Mitomycin C Kyowa Hakko Kirin N/A Dispase II, powder Life Technologies Cat# 17105-041 (Continued on next page) e1 Cell Reports 34, 108715, February 2, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER TrypLETM Express Enzyme (1X), phenol red Thermo Fisher Scientific Cat# 12605010 KnockOutTM Serum Replacement Life Technologies Cat# 10828-028 Sodium pyruvate Life Technologies Cat# 11360-070 Non-essential amino acids Life Technologies Cat# 11140-050 L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin-streptomycin solution Life Technologies Cat# 15140-122 2-Mercaptoethanol Life Technologies Cat# 21985-023 Monothioglycerol Wako Cat# 195-15791 Y-27632 Wako Cat# 034-24024 Recombinant human EGF R&D Systems Cat# 236-EG Recombinant human KGF Wako Cat# 112-00813 Recombinant human TGFa R&D Systems Cat# 239_A Recombinant human Amphiregulin R&D Systems Cat# 262-AR B27TM supplement Life Technologies Cat# 17504-044 QIAzol Lysis Reagent QIAGEN Cat# 79306 4% paraformaldehyde phosphate buffer solution Wako Cat# 163-20145 Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 TritonTM-X 100 Sigma-Aldrich Cat# T8787 Hoechst 33342 Wako Cat# 346-07951 RIPA buffer (10x) Nacalai Tesque Cat# 08714-04 PhosSTOPTM (phosphatase inhibitor) Sigma-Aldrich Cat# 4906845001 StemProTM AccutaseTM Cell Dissociation Reagent Life Technologies Cat# A11105-01 Methanol Wako Cat# 131-01826 10% formaldehyde neutral buffer solution Wako Cat# 062-01661 Rhodamine B Wako Cat# 180-00132 Critical commercial assays SuperScriptTM III First-Strand Synthesis System for qRT-PCR Life Technologies Cat# 18080051 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 ECLTM Prime Western Blotting Detection Reagent GE Healthcare Cat# RPN2236 BD Cytofix/CytopermTM Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 PAS staining kit MERCK KGaA Cat#101646 ELISA Kit for Mucin 5 Subtype AC (MUC5AC) USCN Cat# SEA756Hu Experimental models: cell lines Human: iPS cell line 1383D2 Center for iPS Cell Research and Application N/A Human: iPS cell line 201B7 RIKEN Bio Resource Center Cat# HPS0063; RRID: CVCL_A324 Human: iPS cell line YZWJs524 Center for iPS Cell Research and Application N/A Mouse: NIH 3T3 cell line N/A N/A Oligonucleotides TaqMan probes were listed in Table S1 Life Technologies Cat# 4331182 Software and algorithms AxioVs40 version 4.8.2.0 Carl Zeiss N/A (Continued on next page) Cell Reports 34, 108715, February 2, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER 7500 Software version 2.0.6 Thermo Fisher Scientific RRID: SCR_014596; https://www. thermofisher.com/us/en/home/technicalresources/software-downloads/appliedbiosystems-7500-real-time-pcrsystem.html WorkOut 2.5 version 1.0.0.0 Dazdaq Solutions N/A Image Lab Software version 4.1 Bio-Rad RRID: SCR_014210; http://www.bio-rad. com/en-us/sku/ 1709690-image-lab-software?ID=1709690 Cell Sorter Software version 2.1.5 SONY N/A JMP pro version 14.1.0 SAS RRID: SCR_014242; https://www.jmp.com/ en_us/software/ predictive-analytics-software.html
Quantitative RT-PCR:Article Title: Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-p63 Santa Cruz Biotechnology Cat# sc-8431; RRID: AB_628091; clone 4A4 Rabbit polyclonal anti-PAX6 Covance Research Products Inc Cat# PRB-278P; RRID: AB_291612 Rabbit polyclonal anti-E-cadherin Santa Cruz Biotechnology Cat# sc-7870; RRID: AB_2076666 Goat polyclonal anti-K12 Santa Cruz Biotechnology Cat# sc-17098; RRID: AB_639266 Mouse monoclonal anti-MUC5AC Santa Cruz Biotechnology Cat# sc-33667; RRID: AB_627973; clone CLH2 Mouse monoclonal anti-K13 Abcam Cat# ab16112; RRID: AB_302267; clone AE8 Mouse monoclonal anti-MUC4 Abcam Cat# ab52263; RRID: AB_881163; clone 8G-7 Mouse monoclonal anti-K4 Abcam Cat# ab9004; RRID: AB_306932; clone 6B10 Mouse monoclonal anti-K7 Abcam Cat# ab9021; RRID: AB_306947; clone RCK105 Rabbit polyclonal anti-K19 Abcam Cat# ab15463; RRID: AB_2281021 Mouse monoclonal anti-K10 Dako Cat# M7002; clone DE-K10 Rabbit monoclonal anti-phospho-EGF receptor (Tyr1068) Cell Signaling Technology Cat# 3777; RRID: AB_2096270; clone D7A5 Rabbit monoclonal anti-EGF receptor Cell Signaling Technology Cat# 4267; RRID: AB_2246311; clone D38B1 Mouse monoclonal anti-b-actin Sigma-Aldrich Cat# 5441; RRID: AB_476744; clone AC-15 Mouse monoclonal anti-SSEA-4 PE conjugate BioLegend Cat# 330406; RRID: AB_1089206; clone MC-813-70 Mouse monoclonal anti-CD104 Alexa Fluor 647 conjugate BD Biosciences Cat# 624024; clone 450-9D Mouse monoclonal anti-CD200 PE-Cy7 conjugate BD Biosciences Cat# 624052; clone MRC OX-104 Donkey anti-mouse IgG Alexa Fluor 488 conjugate Life Technologies Cat# A-21202; RRID: AB_141607 Donkey anti-rabbit IgG Alexa Fluor 594 conjugate Life Technologies Cat# A-21207; RRID: AB_141637 Donkey anti-goat IgG Alexa Fluor 568 conjugate Life Technologies Cat# A-11057; RRID: AB_142581 Donkey anti-rabbit IgG horseradish peroxidase conjugate GE Healthcare Cat# NA934-100Ul; RRID: AB_772206 Sheep anti-mouse IgG horseradish peroxidase conjugate GE Healthcare Cat# NA931-100Ul; RRID: AB_772210 Biological samples Research-grade human corneoscleral tissue SightLife https://sightlife.org/ Chemicals, peptides, and recombinant proteins iMatrix-511 (LN511E8) Nippi Cat# 892012 FBS Life Technologies Cat# 12483-020 Mitomycin C Kyowa Hakko Kirin N/A Dispase II, powder Life Technologies Cat# 17105-041 (Continued on next page) e1 Cell Reports 34, 108715, February 2, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER TrypLETM Express Enzyme (1X), phenol red Thermo Fisher Scientific Cat# 12605010 KnockOutTM Serum Replacement Life Technologies Cat# 10828-028 Sodium pyruvate Life Technologies Cat# 11360-070 Non-essential amino acids Life Technologies Cat# 11140-050 L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin-streptomycin solution Life Technologies Cat# 15140-122 2-Mercaptoethanol Life Technologies Cat# 21985-023 Monothioglycerol Wako Cat# 195-15791 Y-27632 Wako Cat# 034-24024 Recombinant human EGF R&D Systems Cat# 236-EG Recombinant human KGF Wako Cat# 112-00813 Recombinant human TGFa R&D Systems Cat# 239_A Recombinant human Amphiregulin R&D Systems Cat# 262-AR B27TM supplement Life Technologies Cat# 17504-044 QIAzol Lysis Reagent QIAGEN Cat# 79306 4% paraformaldehyde phosphate buffer solution Wako Cat# 163-20145 Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 TritonTM-X 100 Sigma-Aldrich Cat# T8787 Hoechst 33342 Wako Cat# 346-07951 RIPA buffer (10x) Nacalai Tesque Cat# 08714-04 PhosSTOPTM (phosphatase inhibitor) Sigma-Aldrich Cat# 4906845001 StemProTM AccutaseTM Cell Dissociation Reagent Life Technologies Cat# A11105-01 Methanol Wako Cat# 131-01826 10% formaldehyde neutral buffer solution Wako Cat# 062-01661 Rhodamine B Wako Cat# 180-00132 Critical commercial assays SuperScriptTM III First-Strand Synthesis System for qRT-PCR Life Technologies Cat# 18080051 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 ECLTM Prime Western Blotting Detection Reagent GE Healthcare Cat# RPN2236 BD Cytofix/CytopermTM Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 PAS staining kit MERCK KGaA Cat#101646 ELISA Kit for Mucin 5 Subtype AC (MUC5AC) USCN Cat# SEA756Hu Experimental models: cell lines Human: iPS cell line 1383D2 Center for iPS Cell Research and Application N/A Human: iPS cell line 201B7 RIKEN Bio Resource Center Cat# HPS0063; RRID: CVCL_A324 Human: iPS cell line YZWJs524 Center for iPS Cell Research and Application N/A Mouse: NIH 3T3 cell line N/A N/A Oligonucleotides TaqMan probes were listed in Table S1 Life Technologies Cat# 4331182 Software and algorithms AxioVs40 version 4.8.2.0 Carl Zeiss N/A (Continued on next page) Cell Reports 34, 108715, February 2, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER 7500 Software version 2.0.6 Thermo Fisher Scientific RRID: SCR_014596; https://www. thermofisher.com/us/en/home/technicalresources/software-downloads/appliedbiosystems-7500-real-time-pcrsystem.html WorkOut 2.5 version 1.0.0.0 Dazdaq Solutions N/A Image Lab Software version 4.1 Bio-Rad RRID: SCR_014210; http://www.bio-rad. com/en-us/sku/ 1709690-image-lab-software?ID=1709690 Cell Sorter Software version 2.1.5 SONY N/A JMP pro version 14.1.0 SAS RRID: SCR_014242; https://www.jmp.com/ en_us/software/ predictive-analytics-software.html
Bicinchoninic Acid Protein Assay:Article Title: Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-p63 Santa Cruz Biotechnology Cat# sc-8431; RRID: AB_628091; clone 4A4 Rabbit polyclonal anti-PAX6 Covance Research Products Inc Cat# PRB-278P; RRID: AB_291612 Rabbit polyclonal anti-E-cadherin Santa Cruz Biotechnology Cat# sc-7870; RRID: AB_2076666 Goat polyclonal anti-K12 Santa Cruz Biotechnology Cat# sc-17098; RRID: AB_639266 Mouse monoclonal anti-MUC5AC Santa Cruz Biotechnology Cat# sc-33667; RRID: AB_627973; clone CLH2 Mouse monoclonal anti-K13 Abcam Cat# ab16112; RRID: AB_302267; clone AE8 Mouse monoclonal anti-MUC4 Abcam Cat# ab52263; RRID: AB_881163; clone 8G-7 Mouse monoclonal anti-K4 Abcam Cat# ab9004; RRID: AB_306932; clone 6B10 Mouse monoclonal anti-K7 Abcam Cat# ab9021; RRID: AB_306947; clone RCK105 Rabbit polyclonal anti-K19 Abcam Cat# ab15463; RRID: AB_2281021 Mouse monoclonal anti-K10 Dako Cat# M7002; clone DE-K10 Rabbit monoclonal anti-phospho-EGF receptor (Tyr1068) Cell Signaling Technology Cat# 3777; RRID: AB_2096270; clone D7A5 Rabbit monoclonal anti-EGF receptor Cell Signaling Technology Cat# 4267; RRID: AB_2246311; clone D38B1 Mouse monoclonal anti-b-actin Sigma-Aldrich Cat# 5441; RRID: AB_476744; clone AC-15 Mouse monoclonal anti-SSEA-4 PE conjugate BioLegend Cat# 330406; RRID: AB_1089206; clone MC-813-70 Mouse monoclonal anti-CD104 Alexa Fluor 647 conjugate BD Biosciences Cat# 624024; clone 450-9D Mouse monoclonal anti-CD200 PE-Cy7 conjugate BD Biosciences Cat# 624052; clone MRC OX-104 Donkey anti-mouse IgG Alexa Fluor 488 conjugate Life Technologies Cat# A-21202; RRID: AB_141607 Donkey anti-rabbit IgG Alexa Fluor 594 conjugate Life Technologies Cat# A-21207; RRID: AB_141637 Donkey anti-goat IgG Alexa Fluor 568 conjugate Life Technologies Cat# A-11057; RRID: AB_142581 Donkey anti-rabbit IgG horseradish peroxidase conjugate GE Healthcare Cat# NA934-100Ul; RRID: AB_772206 Sheep anti-mouse IgG horseradish peroxidase conjugate GE Healthcare Cat# NA931-100Ul; RRID: AB_772210 Biological samples Research-grade human corneoscleral tissue SightLife https://sightlife.org/ Chemicals, peptides, and recombinant proteins iMatrix-511 (LN511E8) Nippi Cat# 892012 FBS Life Technologies Cat# 12483-020 Mitomycin C Kyowa Hakko Kirin N/A Dispase II, powder Life Technologies Cat# 17105-041 (Continued on next page) e1 Cell Reports 34, 108715, February 2, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER TrypLETM Express Enzyme (1X), phenol red Thermo Fisher Scientific Cat# 12605010 KnockOutTM Serum Replacement Life Technologies Cat# 10828-028 Sodium pyruvate Life Technologies Cat# 11360-070 Non-essential amino acids Life Technologies Cat# 11140-050 L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin-streptomycin solution Life Technologies Cat# 15140-122 2-Mercaptoethanol Life Technologies Cat# 21985-023 Monothioglycerol Wako Cat# 195-15791 Y-27632 Wako Cat# 034-24024 Recombinant human EGF R&D Systems Cat# 236-EG Recombinant human KGF Wako Cat# 112-00813 Recombinant human TGFa R&D Systems Cat# 239_A Recombinant human Amphiregulin R&D Systems Cat# 262-AR B27TM supplement Life Technologies Cat# 17504-044 QIAzol Lysis Reagent QIAGEN Cat# 79306 4% paraformaldehyde phosphate buffer solution Wako Cat# 163-20145 Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 TritonTM-X 100 Sigma-Aldrich Cat# T8787 Hoechst 33342 Wako Cat# 346-07951 RIPA buffer (10x) Nacalai Tesque Cat# 08714-04 PhosSTOPTM (phosphatase inhibitor) Sigma-Aldrich Cat# 4906845001 StemProTM AccutaseTM Cell Dissociation Reagent Life Technologies Cat# A11105-01 Methanol Wako Cat# 131-01826 10% formaldehyde neutral buffer solution Wako Cat# 062-01661 Rhodamine B Wako Cat# 180-00132 Critical commercial assays SuperScriptTM III First-Strand Synthesis System for qRT-PCR Life Technologies Cat# 18080051 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 ECLTM Prime Western Blotting Detection Reagent GE Healthcare Cat# RPN2236 BD Cytofix/CytopermTM Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 PAS staining kit MERCK KGaA Cat#101646 ELISA Kit for Mucin 5 Subtype AC (MUC5AC) USCN Cat# SEA756Hu Experimental models: cell lines Human: iPS cell line 1383D2 Center for iPS Cell Research and Application N/A Human: iPS cell line 201B7 RIKEN Bio Resource Center Cat# HPS0063; RRID: CVCL_A324 Human: iPS cell line YZWJs524 Center for iPS Cell Research and Application N/A Mouse: NIH 3T3 cell line N/A N/A Oligonucleotides TaqMan probes were listed in Table S1 Life Technologies Cat# 4331182 Software and algorithms AxioVs40 version 4.8.2.0 Carl Zeiss N/A (Continued on next page) Cell Reports 34, 108715, February 2, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER 7500 Software version 2.0.6 Thermo Fisher Scientific RRID: SCR_014596; https://www. thermofisher.com/us/en/home/technicalresources/software-downloads/appliedbiosystems-7500-real-time-pcrsystem.html WorkOut 2.5 version 1.0.0.0 Dazdaq Solutions N/A Image Lab Software version 4.1 Bio-Rad RRID: SCR_014210; http://www.bio-rad. com/en-us/sku/ 1709690-image-lab-software?ID=1709690 Cell Sorter Software version 2.1.5 SONY N/A JMP pro version 14.1.0 SAS RRID: SCR_014242; https://www.jmp.com/ en_us/software/ predictive-analytics-software.html
Western Blot:Article Title: Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-p63 Santa Cruz Biotechnology Cat# sc-8431; RRID: AB_628091; clone 4A4 Rabbit polyclonal anti-PAX6 Covance Research Products Inc Cat# PRB-278P; RRID: AB_291612 Rabbit polyclonal anti-E-cadherin Santa Cruz Biotechnology Cat# sc-7870; RRID: AB_2076666 Goat polyclonal anti-K12 Santa Cruz Biotechnology Cat# sc-17098; RRID: AB_639266 Mouse monoclonal anti-MUC5AC Santa Cruz Biotechnology Cat# sc-33667; RRID: AB_627973; clone CLH2 Mouse monoclonal anti-K13 Abcam Cat# ab16112; RRID: AB_302267; clone AE8 Mouse monoclonal anti-MUC4 Abcam Cat# ab52263; RRID: AB_881163; clone 8G-7 Mouse monoclonal anti-K4 Abcam Cat# ab9004; RRID: AB_306932; clone 6B10 Mouse monoclonal anti-K7 Abcam Cat# ab9021; RRID: AB_306947; clone RCK105 Rabbit polyclonal anti-K19 Abcam Cat# ab15463; RRID: AB_2281021 Mouse monoclonal anti-K10 Dako Cat# M7002; clone DE-K10 Rabbit monoclonal anti-phospho-EGF receptor (Tyr1068) Cell Signaling Technology Cat# 3777; RRID: AB_2096270; clone D7A5 Rabbit monoclonal anti-EGF receptor Cell Signaling Technology Cat# 4267; RRID: AB_2246311; clone D38B1 Mouse monoclonal anti-b-actin Sigma-Aldrich Cat# 5441; RRID: AB_476744; clone AC-15 Mouse monoclonal anti-SSEA-4 PE conjugate BioLegend Cat# 330406; RRID: AB_1089206; clone MC-813-70 Mouse monoclonal anti-CD104 Alexa Fluor 647 conjugate BD Biosciences Cat# 624024; clone 450-9D Mouse monoclonal anti-CD200 PE-Cy7 conjugate BD Biosciences Cat# 624052; clone MRC OX-104 Donkey anti-mouse IgG Alexa Fluor 488 conjugate Life Technologies Cat# A-21202; RRID: AB_141607 Donkey anti-rabbit IgG Alexa Fluor 594 conjugate Life Technologies Cat# A-21207; RRID: AB_141637 Donkey anti-goat IgG Alexa Fluor 568 conjugate Life Technologies Cat# A-11057; RRID: AB_142581 Donkey anti-rabbit IgG horseradish peroxidase conjugate GE Healthcare Cat# NA934-100Ul; RRID: AB_772206 Sheep anti-mouse IgG horseradish peroxidase conjugate GE Healthcare Cat# NA931-100Ul; RRID: AB_772210 Biological samples Research-grade human corneoscleral tissue SightLife https://sightlife.org/ Chemicals, peptides, and recombinant proteins iMatrix-511 (LN511E8) Nippi Cat# 892012 FBS Life Technologies Cat# 12483-020 Mitomycin C Kyowa Hakko Kirin N/A Dispase II, powder Life Technologies Cat# 17105-041 (Continued on next page) e1 Cell Reports 34, 108715, February 2, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER TrypLETM Express Enzyme (1X), phenol red Thermo Fisher Scientific Cat# 12605010 KnockOutTM Serum Replacement Life Technologies Cat# 10828-028 Sodium pyruvate Life Technologies Cat# 11360-070 Non-essential amino acids Life Technologies Cat# 11140-050 L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin-streptomycin solution Life Technologies Cat# 15140-122 2-Mercaptoethanol Life Technologies Cat# 21985-023 Monothioglycerol Wako Cat# 195-15791 Y-27632 Wako Cat# 034-24024 Recombinant human EGF R&D Systems Cat# 236-EG Recombinant human KGF Wako Cat# 112-00813 Recombinant human TGFa R&D Systems Cat# 239_A Recombinant human Amphiregulin R&D Systems Cat# 262-AR B27TM supplement Life Technologies Cat# 17504-044 QIAzol Lysis Reagent QIAGEN Cat# 79306 4% paraformaldehyde phosphate buffer solution Wako Cat# 163-20145 Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 TritonTM-X 100 Sigma-Aldrich Cat# T8787 Hoechst 33342 Wako Cat# 346-07951 RIPA buffer (10x) Nacalai Tesque Cat# 08714-04 PhosSTOPTM (phosphatase inhibitor) Sigma-Aldrich Cat# 4906845001 StemProTM AccutaseTM Cell Dissociation Reagent Life Technologies Cat# A11105-01 Methanol Wako Cat# 131-01826 10% formaldehyde neutral buffer solution Wako Cat# 062-01661 Rhodamine B Wako Cat# 180-00132 Critical commercial assays SuperScriptTM III First-Strand Synthesis System for qRT-PCR Life Technologies Cat# 18080051 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 ECLTM Prime Western Blotting Detection Reagent GE Healthcare Cat# RPN2236 BD Cytofix/CytopermTM Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 PAS staining kit MERCK KGaA Cat#101646 ELISA Kit for Mucin 5 Subtype AC (MUC5AC) USCN Cat# SEA756Hu Experimental models: cell lines Human: iPS cell line 1383D2 Center for iPS Cell Research and Application N/A Human: iPS cell line 201B7 RIKEN Bio Resource Center Cat# HPS0063; RRID: CVCL_A324 Human: iPS cell line YZWJs524 Center for iPS Cell Research and Application N/A Mouse: NIH 3T3 cell line N/A N/A Oligonucleotides TaqMan probes were listed in Table S1 Life Technologies Cat# 4331182 Software and algorithms AxioVs40 version 4.8.2.0 Carl Zeiss N/A (Continued on next page) Cell Reports 34, 108715, February 2, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER 7500 Software version 2.0.6 Thermo Fisher Scientific RRID: SCR_014596; https://www. thermofisher.com/us/en/home/technicalresources/software-downloads/appliedbiosystems-7500-real-time-pcrsystem.html WorkOut 2.5 version 1.0.0.0 Dazdaq Solutions N/A Image Lab Software version 4.1 Bio-Rad RRID: SCR_014210; http://www.bio-rad. com/en-us/sku/ 1709690-image-lab-software?ID=1709690 Cell Sorter Software version 2.1.5 SONY N/A JMP pro version 14.1.0 SAS RRID: SCR_014242; https://www.jmp.com/ en_us/software/ predictive-analytics-software.html
Staining:Article Title: Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-p63 Santa Cruz Biotechnology Cat# sc-8431; RRID: AB_628091; clone 4A4 Rabbit polyclonal anti-PAX6 Covance Research Products Inc Cat# PRB-278P; RRID: AB_291612 Rabbit polyclonal anti-E-cadherin Santa Cruz Biotechnology Cat# sc-7870; RRID: AB_2076666 Goat polyclonal anti-K12 Santa Cruz Biotechnology Cat# sc-17098; RRID: AB_639266 Mouse monoclonal anti-MUC5AC Santa Cruz Biotechnology Cat# sc-33667; RRID: AB_627973; clone CLH2 Mouse monoclonal anti-K13 Abcam Cat# ab16112; RRID: AB_302267; clone AE8 Mouse monoclonal anti-MUC4 Abcam Cat# ab52263; RRID: AB_881163; clone 8G-7 Mouse monoclonal anti-K4 Abcam Cat# ab9004; RRID: AB_306932; clone 6B10 Mouse monoclonal anti-K7 Abcam Cat# ab9021; RRID: AB_306947; clone RCK105 Rabbit polyclonal anti-K19 Abcam Cat# ab15463; RRID: AB_2281021 Mouse monoclonal anti-K10 Dako Cat# M7002; clone DE-K10 Rabbit monoclonal anti-phospho-EGF receptor (Tyr1068) Cell Signaling Technology Cat# 3777; RRID: AB_2096270; clone D7A5 Rabbit monoclonal anti-EGF receptor Cell Signaling Technology Cat# 4267; RRID: AB_2246311; clone D38B1 Mouse monoclonal anti-b-actin Sigma-Aldrich Cat# 5441; RRID: AB_476744; clone AC-15 Mouse monoclonal anti-SSEA-4 PE conjugate BioLegend Cat# 330406; RRID: AB_1089206; clone MC-813-70 Mouse monoclonal anti-CD104 Alexa Fluor 647 conjugate BD Biosciences Cat# 624024; clone 450-9D Mouse monoclonal anti-CD200 PE-Cy7 conjugate BD Biosciences Cat# 624052; clone MRC OX-104 Donkey anti-mouse IgG Alexa Fluor 488 conjugate Life Technologies Cat# A-21202; RRID: AB_141607 Donkey anti-rabbit IgG Alexa Fluor 594 conjugate Life Technologies Cat# A-21207; RRID: AB_141637 Donkey anti-goat IgG Alexa Fluor 568 conjugate Life Technologies Cat# A-11057; RRID: AB_142581 Donkey anti-rabbit IgG horseradish peroxidase conjugate GE Healthcare Cat# NA934-100Ul; RRID: AB_772206 Sheep anti-mouse IgG horseradish peroxidase conjugate GE Healthcare Cat# NA931-100Ul; RRID: AB_772210 Biological samples Research-grade human corneoscleral tissue SightLife https://sightlife.org/ Chemicals, peptides, and recombinant proteins iMatrix-511 (LN511E8) Nippi Cat# 892012 FBS Life Technologies Cat# 12483-020 Mitomycin C Kyowa Hakko Kirin N/A Dispase II, powder Life Technologies Cat# 17105-041 (Continued on next page) e1 Cell Reports 34, 108715, February 2, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER TrypLETM Express Enzyme (1X), phenol red Thermo Fisher Scientific Cat# 12605010 KnockOutTM Serum Replacement Life Technologies Cat# 10828-028 Sodium pyruvate Life Technologies Cat# 11360-070 Non-essential amino acids Life Technologies Cat# 11140-050 L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin-streptomycin solution Life Technologies Cat# 15140-122 2-Mercaptoethanol Life Technologies Cat# 21985-023 Monothioglycerol Wako Cat# 195-15791 Y-27632 Wako Cat# 034-24024 Recombinant human EGF R&D Systems Cat# 236-EG Recombinant human KGF Wako Cat# 112-00813 Recombinant human TGFa R&D Systems Cat# 239_A Recombinant human Amphiregulin R&D Systems Cat# 262-AR B27TM supplement Life Technologies Cat# 17504-044 QIAzol Lysis Reagent QIAGEN Cat# 79306 4% paraformaldehyde phosphate buffer solution Wako Cat# 163-20145 Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 TritonTM-X 100 Sigma-Aldrich Cat# T8787 Hoechst 33342 Wako Cat# 346-07951 RIPA buffer (10x) Nacalai Tesque Cat# 08714-04 PhosSTOPTM (phosphatase inhibitor) Sigma-Aldrich Cat# 4906845001 StemProTM AccutaseTM Cell Dissociation Reagent Life Technologies Cat# A11105-01 Methanol Wako Cat# 131-01826 10% formaldehyde neutral buffer solution Wako Cat# 062-01661 Rhodamine B Wako Cat# 180-00132 Critical commercial assays SuperScriptTM III First-Strand Synthesis System for qRT-PCR Life Technologies Cat# 18080051 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 ECLTM Prime Western Blotting Detection Reagent GE Healthcare Cat# RPN2236 BD Cytofix/CytopermTM Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 PAS staining kit MERCK KGaA Cat#101646 ELISA Kit for Mucin 5 Subtype AC (MUC5AC) USCN Cat# SEA756Hu Experimental models: cell lines Human: iPS cell line 1383D2 Center for iPS Cell Research and Application N/A Human: iPS cell line 201B7 RIKEN Bio Resource Center Cat# HPS0063; RRID: CVCL_A324 Human: iPS cell line YZWJs524 Center for iPS Cell Research and Application N/A Mouse: NIH 3T3 cell line N/A N/A Oligonucleotides TaqMan probes were listed in Table S1 Life Technologies Cat# 4331182 Software and algorithms AxioVs40 version 4.8.2.0 Carl Zeiss N/A (Continued on next page) Cell Reports 34, 108715, February 2, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER 7500 Software version 2.0.6 Thermo Fisher Scientific RRID: SCR_014596; https://www. thermofisher.com/us/en/home/technicalresources/software-downloads/appliedbiosystems-7500-real-time-pcrsystem.html WorkOut 2.5 version 1.0.0.0 Dazdaq Solutions N/A Image Lab Software version 4.1 Bio-Rad RRID: SCR_014210; http://www.bio-rad. com/en-us/sku/ 1709690-image-lab-software?ID=1709690 Cell Sorter Software version 2.1.5 SONY N/A JMP pro version 14.1.0 SAS RRID: SCR_014242; https://www.jmp.com/ en_us/software/ predictive-analytics-software.html
Enzyme-linked Immunosorbent Assay:Article Title: Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-p63 Santa Cruz Biotechnology Cat# sc-8431; RRID: AB_628091; clone 4A4 Rabbit polyclonal anti-PAX6 Covance Research Products Inc Cat# PRB-278P; RRID: AB_291612 Rabbit polyclonal anti-E-cadherin Santa Cruz Biotechnology Cat# sc-7870; RRID: AB_2076666 Goat polyclonal anti-K12 Santa Cruz Biotechnology Cat# sc-17098; RRID: AB_639266 Mouse monoclonal anti-MUC5AC Santa Cruz Biotechnology Cat# sc-33667; RRID: AB_627973; clone CLH2 Mouse monoclonal anti-K13 Abcam Cat# ab16112; RRID: AB_302267; clone AE8 Mouse monoclonal anti-MUC4 Abcam Cat# ab52263; RRID: AB_881163; clone 8G-7 Mouse monoclonal anti-K4 Abcam Cat# ab9004; RRID: AB_306932; clone 6B10 Mouse monoclonal anti-K7 Abcam Cat# ab9021; RRID: AB_306947; clone RCK105 Rabbit polyclonal anti-K19 Abcam Cat# ab15463; RRID: AB_2281021 Mouse monoclonal anti-K10 Dako Cat# M7002; clone DE-K10 Rabbit monoclonal anti-phospho-EGF receptor (Tyr1068) Cell Signaling Technology Cat# 3777; RRID: AB_2096270; clone D7A5 Rabbit monoclonal anti-EGF receptor Cell Signaling Technology Cat# 4267; RRID: AB_2246311; clone D38B1 Mouse monoclonal anti-b-actin Sigma-Aldrich Cat# 5441; RRID: AB_476744; clone AC-15 Mouse monoclonal anti-SSEA-4 PE conjugate BioLegend Cat# 330406; RRID: AB_1089206; clone MC-813-70 Mouse monoclonal anti-CD104 Alexa Fluor 647 conjugate BD Biosciences Cat# 624024; clone 450-9D Mouse monoclonal anti-CD200 PE-Cy7 conjugate BD Biosciences Cat# 624052; clone MRC OX-104 Donkey anti-mouse IgG Alexa Fluor 488 conjugate Life Technologies Cat# A-21202; RRID: AB_141607 Donkey anti-rabbit IgG Alexa Fluor 594 conjugate Life Technologies Cat# A-21207; RRID: AB_141637 Donkey anti-goat IgG Alexa Fluor 568 conjugate Life Technologies Cat# A-11057; RRID: AB_142581 Donkey anti-rabbit IgG horseradish peroxidase conjugate GE Healthcare Cat# NA934-100Ul; RRID: AB_772206 Sheep anti-mouse IgG horseradish peroxidase conjugate GE Healthcare Cat# NA931-100Ul; RRID: AB_772210 Biological samples Research-grade human corneoscleral tissue SightLife https://sightlife.org/ Chemicals, peptides, and recombinant proteins iMatrix-511 (LN511E8) Nippi Cat# 892012 FBS Life Technologies Cat# 12483-020 Mitomycin C Kyowa Hakko Kirin N/A Dispase II, powder Life Technologies Cat# 17105-041 (Continued on next page) e1 Cell Reports 34, 108715, February 2, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER TrypLETM Express Enzyme (1X), phenol red Thermo Fisher Scientific Cat# 12605010 KnockOutTM Serum Replacement Life Technologies Cat# 10828-028 Sodium pyruvate Life Technologies Cat# 11360-070 Non-essential amino acids Life Technologies Cat# 11140-050 L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin-streptomycin solution Life Technologies Cat# 15140-122 2-Mercaptoethanol Life Technologies Cat# 21985-023 Monothioglycerol Wako Cat# 195-15791 Y-27632 Wako Cat# 034-24024 Recombinant human EGF R&D Systems Cat# 236-EG Recombinant human KGF Wako Cat# 112-00813 Recombinant human TGFa R&D Systems Cat# 239_A Recombinant human Amphiregulin R&D Systems Cat# 262-AR B27TM supplement Life Technologies Cat# 17504-044 QIAzol Lysis Reagent QIAGEN Cat# 79306 4% paraformaldehyde phosphate buffer solution Wako Cat# 163-20145 Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 TritonTM-X 100 Sigma-Aldrich Cat# T8787 Hoechst 33342 Wako Cat# 346-07951 RIPA buffer (10x) Nacalai Tesque Cat# 08714-04 PhosSTOPTM (phosphatase inhibitor) Sigma-Aldrich Cat# 4906845001 StemProTM AccutaseTM Cell Dissociation Reagent Life Technologies Cat# A11105-01 Methanol Wako Cat# 131-01826 10% formaldehyde neutral buffer solution Wako Cat# 062-01661 Rhodamine B Wako Cat# 180-00132 Critical commercial assays SuperScriptTM III First-Strand Synthesis System for qRT-PCR Life Technologies Cat# 18080051 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 ECLTM Prime Western Blotting Detection Reagent GE Healthcare Cat# RPN2236 BD Cytofix/CytopermTM Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 PAS staining kit MERCK KGaA Cat#101646 ELISA Kit for Mucin 5 Subtype AC (MUC5AC) USCN Cat# SEA756Hu Experimental models: cell lines Human: iPS cell line 1383D2 Center for iPS Cell Research and Application N/A Human: iPS cell line 201B7 RIKEN Bio Resource Center Cat# HPS0063; RRID: CVCL_A324 Human: iPS cell line YZWJs524 Center for iPS Cell Research and Application N/A Mouse: NIH 3T3 cell line N/A N/A Oligonucleotides TaqMan probes were listed in Table S1 Life Technologies Cat# 4331182 Software and algorithms AxioVs40 version 4.8.2.0 Carl Zeiss N/A (Continued on next page) Cell Reports 34, 108715, February 2, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER 7500 Software version 2.0.6 Thermo Fisher Scientific RRID: SCR_014596; https://www. thermofisher.com/us/en/home/technicalresources/software-downloads/appliedbiosystems-7500-real-time-pcrsystem.html WorkOut 2.5 version 1.0.0.0 Dazdaq Solutions N/A Image Lab Software version 4.1 Bio-Rad RRID: SCR_014210; http://www.bio-rad. com/en-us/sku/ 1709690-image-lab-software?ID=1709690 Cell Sorter Software version 2.1.5 SONY N/A JMP pro version 14.1.0 SAS RRID: SCR_014242; https://www.jmp.com/ en_us/software/ predictive-analytics-software.html
Software:Article Title: Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-p63 Santa Cruz Biotechnology Cat# sc-8431; RRID: AB_628091; clone 4A4 Rabbit polyclonal anti-PAX6 Covance Research Products Inc Cat# PRB-278P; RRID: AB_291612 Rabbit polyclonal anti-E-cadherin Santa Cruz Biotechnology Cat# sc-7870; RRID: AB_2076666 Goat polyclonal anti-K12 Santa Cruz Biotechnology Cat# sc-17098; RRID: AB_639266 Mouse monoclonal anti-MUC5AC Santa Cruz Biotechnology Cat# sc-33667; RRID: AB_627973; clone CLH2 Mouse monoclonal anti-K13 Abcam Cat# ab16112; RRID: AB_302267; clone AE8 Mouse monoclonal anti-MUC4 Abcam Cat# ab52263; RRID: AB_881163; clone 8G-7 Mouse monoclonal anti-K4 Abcam Cat# ab9004; RRID: AB_306932; clone 6B10 Mouse monoclonal anti-K7 Abcam Cat# ab9021; RRID: AB_306947; clone RCK105 Rabbit polyclonal anti-K19 Abcam Cat# ab15463; RRID: AB_2281021 Mouse monoclonal anti-K10 Dako Cat# M7002; clone DE-K10 Rabbit monoclonal anti-phospho-EGF receptor (Tyr1068) Cell Signaling Technology Cat# 3777; RRID: AB_2096270; clone D7A5 Rabbit monoclonal anti-EGF receptor Cell Signaling Technology Cat# 4267; RRID: AB_2246311; clone D38B1 Mouse monoclonal anti-b-actin Sigma-Aldrich Cat# 5441; RRID: AB_476744; clone AC-15 Mouse monoclonal anti-SSEA-4 PE conjugate BioLegend Cat# 330406; RRID: AB_1089206; clone MC-813-70 Mouse monoclonal anti-CD104 Alexa Fluor 647 conjugate BD Biosciences Cat# 624024; clone 450-9D Mouse monoclonal anti-CD200 PE-Cy7 conjugate BD Biosciences Cat# 624052; clone MRC OX-104 Donkey anti-mouse IgG Alexa Fluor 488 conjugate Life Technologies Cat# A-21202; RRID: AB_141607 Donkey anti-rabbit IgG Alexa Fluor 594 conjugate Life Technologies Cat# A-21207; RRID: AB_141637 Donkey anti-goat IgG Alexa Fluor 568 conjugate Life Technologies Cat# A-11057; RRID: AB_142581 Donkey anti-rabbit IgG horseradish peroxidase conjugate GE Healthcare Cat# NA934-100Ul; RRID: AB_772206 Sheep anti-mouse IgG horseradish peroxidase conjugate GE Healthcare Cat# NA931-100Ul; RRID: AB_772210 Biological samples Research-grade human corneoscleral tissue SightLife https://sightlife.org/ Chemicals, peptides, and recombinant proteins iMatrix-511 (LN511E8) Nippi Cat# 892012 FBS Life Technologies Cat# 12483-020 Mitomycin C Kyowa Hakko Kirin N/A Dispase II, powder Life Technologies Cat# 17105-041 (Continued on next page) e1 Cell Reports 34, 108715, February 2, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER TrypLETM Express Enzyme (1X), phenol red Thermo Fisher Scientific Cat# 12605010 KnockOutTM Serum Replacement Life Technologies Cat# 10828-028 Sodium pyruvate Life Technologies Cat# 11360-070 Non-essential amino acids Life Technologies Cat# 11140-050 L-glutamine Thermo Fisher Scientific Cat# 25030081 Penicillin-streptomycin solution Life Technologies Cat# 15140-122 2-Mercaptoethanol Life Technologies Cat# 21985-023 Monothioglycerol Wako Cat# 195-15791 Y-27632 Wako Cat# 034-24024 Recombinant human EGF R&D Systems Cat# 236-EG Recombinant human KGF Wako Cat# 112-00813 Recombinant human TGFa R&D Systems Cat# 239_A Recombinant human Amphiregulin R&D Systems Cat# 262-AR B27TM supplement Life Technologies Cat# 17504-044 QIAzol Lysis Reagent QIAGEN Cat# 79306 4% paraformaldehyde phosphate buffer solution Wako Cat# 163-20145 Normal donkey serum Jackson ImmunoResearch Cat# 017-000-121 TritonTM-X 100 Sigma-Aldrich Cat# T8787 Hoechst 33342 Wako Cat# 346-07951 RIPA buffer (10x) Nacalai Tesque Cat# 08714-04 PhosSTOPTM (phosphatase inhibitor) Sigma-Aldrich Cat# 4906845001 StemProTM AccutaseTM Cell Dissociation Reagent Life Technologies Cat# A11105-01 Methanol Wako Cat# 131-01826 10% formaldehyde neutral buffer solution Wako Cat# 062-01661 Rhodamine B Wako Cat# 180-00132 Critical commercial assays SuperScriptTM III First-Strand Synthesis System for qRT-PCR Life Technologies Cat# 18080051 PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 ECLTM Prime Western Blotting Detection Reagent GE Healthcare Cat# RPN2236 BD Cytofix/CytopermTM Fixation/ Permeabilization Solution Kit BD Biosciences Cat# 554714 PAS staining kit MERCK KGaA Cat#101646 ELISA Kit for Mucin 5 Subtype AC (MUC5AC) USCN Cat# SEA756Hu Experimental models: cell lines Human: iPS cell line 1383D2 Center for iPS Cell Research and Application N/A Human: iPS cell line 201B7 RIKEN Bio Resource Center Cat# HPS0063; RRID: CVCL_A324 Human: iPS cell line YZWJs524 Center for iPS Cell Research and Application N/A Mouse: NIH 3T3 cell line N/A N/A Oligonucleotides TaqMan probes were listed in Table S1 Life Technologies Cat# 4331182 Software and algorithms AxioVs40 version 4.8.2.0 Carl Zeiss N/A (Continued on next page) Cell Reports 34, 108715, February 2, 2021 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER 7500 Software version 2.0.6 Thermo Fisher Scientific RRID: SCR_014596; https://www. thermofisher.com/us/en/home/technicalresources/software-downloads/appliedbiosystems-7500-real-time-pcrsystem.html WorkOut 2.5 version 1.0.0.0 Dazdaq Solutions N/A Image Lab Software version 4.1 Bio-Rad RRID: SCR_014210; http://www.bio-rad. com/en-us/sku/ 1709690-image-lab-software?ID=1709690 Cell Sorter Software version 2.1.5 SONY N/A JMP pro version 14.1.0 SAS RRID: SCR_014242; https://www.jmp.com/ en_us/software/ predictive-analytics-software.html
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